Cellulases from Chaetomium thermophile var. dissitum
✍ Scribed by Jonny ERIKSEN; Jostein GOKSØYR
- Book ID
- 115116137
- Publisher
- John Wiley and Sons
- Year
- 1977
- Tongue
- English
- Weight
- 464 KB
- Volume
- 77
- Category
- Article
- ISSN
- 1432-1327
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An endocellulase (1-34)~P-D-glucan 4-glucanohydrolase was isolated from the culture filtrates of Chaetomium thermophile. The enzyme was homogeneous by PAGE and SDS-PAGE. The molecular weight was 36 000 by SDS-PAGE and 38 000 by gel filtration. It was a glycoprotein. From the amino acid composition,
## Abstract The ability of __Chaetomium thermophile var. coprophile__ COONEY and EMERSON to utilize carbohydrates as carbon sources for growth has been studied in stationary liquid medium at 45 °C. Among the monosaccharides, the highest growth was obtained on mannose, followed by xylose, glucose, a
An endo-B-glucanase of C.cellulolyticum was purified by a procedure involving concanavalin A (Con A)-Sepharose chromatography and polyacrylamide gel electrophoresis (PAGE). The enzyme produced G1 and oligosaccharides from CMC. Chromatography on Procion Red HE3B-Agarose proved to be useful in the sep