Cellulase production by mixed fungi in solid-substrate fermentation of bagasse

✍ Scribed by R. Dueñas; R. P. Tengerdy; M. Gutierrez-Correa

• Publisher: Springer
• Year: 1995
• Tongue: English
• Weight: 509 KB
• Volume: 11
• Category: Article
• ISSN: 1573-0972
• DOI: 10.1007/bf00367112

✦ Synopsis

Ammonia-treated

bagasse with 80% (w/w) moisture content was subjected to mixed-culture solid-substrate fermentation (SSF) with Tric~odernzu reesei LM-UC4 and Aspergih phenicis QM329, in flask or pot fermenters, for cellulase production.

Significantly higher activities of all the enzymes of the cellulase complex were achieved in 4 days of mixed-culture SSF than in single-culture ('I. reesei) SSF. The highest filter-paper-cellulase and /3glucosidase activities seen in mixed-culture SSF were 18.7 and 38.6 IU/g dry wt, respectively, representing approx. 3-and 6-fold increases over the activities attained in single-culture SSF. The mixed-culture SSF process also converted about 46% of the cellulose and hemicellulose to reducing sugars and enriched the product with 13% fungal protein. The biomass productivity, 0.29 gl-I. h, and enzyme productivity, 28.0 IU I-l.h, were about twice as high in the mixed-culture than in the single-culture.