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Cellular reactions against Burkitt lymphoma cells. I. Colony inhibition with effector cells from patients with Burkitt's lymphoma

✍ Scribed by John F. Hewetson; Sidney H. Golub; George Klein; Surjit Singh

Publisher
John Wiley and Sons
Year
1972
Tongue
French
Weight
577 KB
Volume
10
Category
Article
ISSN
0020-7136

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✦ Synopsis

Abstract

A micro‐assay colony inhibition (CI) test has been developed for studying cell‐mediated reactions against lymphoblastoid target cells. The assay makes use of the ability of the target cells, but not the effector cells, to grow in discrete colonies in semi‐solid medium. Peripheral blood leukocytes from patients with Burkitt's lymphoma and nasopharyngeal carcinoma, and from controls, were used as effector cells in this assay against the Burkitt lymphoma derived lymphoblastoid cell line P3HR‐I. Only five of the 62 samples from patients with these diseases inhibited colony formation by more than 30%. When phytohemagglutinin‐stimulated cells were used in the CI test, colony formation was greatly reduced. CI activity of PHA‐stimulated cells appeared to be correlated to the degree of stimulation, as determined by incorporation of tritiated thymidine. Preliminary data indicate that lymph‐node cells, but not peripheral leukocytes, tested against autochthonous and allogeneic cell lines, are capable of significant colony inhibition.

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