✦ LIBER ✦

Cellular and metabolic specificity in the interaction of adhesion proteins with collagen and with cells

✍ Scribed by Kleinman, H. K. ;Hewitt, A. T. ;Murray, J. C. ;Liotta, L. A. ;Rennard, S. I. ;Pennypacker, J. P. ;McGoodwin, E. B. ;Martin, G. R. ;Fishman, P. H.

Publisher: Wiley (John Wiley & Sons)
Year: 1979
Tongue: English
Weight: 535 KB
Volume: 11
Category: Article
ISSN: 0091-7419
DOI: 10.1002/jss.400110108

No coin nor oath required. For personal study only.

✦ Synopsis

Fibronectin mediates the adhesion of fibroblasts to collagen substrates, binding first to the collagen and then to the cells. We report here that the interaction of the cells with the fibronectin-collagen complex is blocked by specific gangliosides, GD1 a and GT1 , and that the sugar moieties of these gangliosides contain the inhibitory activity. The gangliosides act by binding to fibronectin, suggesting that they may be the cell surface receptor for fibronectin. ment exist for chondrocytes, epidermal cells, and transformed tumorigenic cells, since adhesion of these cells is not stimulated by fibronectin. Chondrocytes adhere via a serum factor that is more temperature-sensitive and less basic than fibronectin. Unlike that of fibroblasts chondrocyte adhesion is stimulated by low levels of gangliosides. Epidermal cells adhere preferentially to type IV (basement membrane) collagen but at a much slower rate than fibroblasts or chondrocytes. This suggests that these epidermal cells synthesize their own specific adhesion factor. Metastatic cells cultured from the T241 fibrosarcoma adhere rapidly to type IV collagen in the absence of fibronectin and do not synthesize significant amounts of collagen or fibronectin. Their growth, in contrast to that of normal fibroblasts, is unaffected by a specific inhibitor of collagen synthesis. These data indicate the importance of specific collagens and adhesion proteins in the adhesion of certain cells and suggest that a reduction in the synthesis of collagen and of fibronectin is related to some of the abnormalities observed in transformed cells.

Evidence is presented that other adhesion proteins or mechanisms of attach-

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