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Cell/surface interactions and adhesion on Ti-6Al-4V: Effects of surface texture

✍ Scribed by J. Chen; S. Mwenifumbo; C. Langhammer; J.-P. McGovern; M. Li; A. Beye; W. O. Soboyejo

Publisher
John Wiley and Sons
Year
2007
Tongue
English
Weight
752 KB
Volume
82B
Category
Article
ISSN
1552-4973

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✦ Synopsis

Abstract

This paper presents the results of an experimental study of the effects of surface texture on the interactions between human osteo‐sarcoma (HOS) cells and Ti‐6Al‐4V. These include the Ti‐6Al‐4V with polished (smooth); Al~2~O~3~ blasted (rough); and laser micro‐grooved geometries with controlled spacings and depths. Immuno‐fluorescence staining of adhesion proteins (actin and vinculin) was used to study the spreading and adhesion of HOS cells in 2 day culture experiments. Quantitative measures of adhesion were also obtained using an enzymatic detachment assay. The results are discussed within the context of existing theories of cell adhesion. The implications of the results are also examined for the design of textured surfaces in biomedical systems. © 2007 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2007

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