In vitro interaction between surface-treated Ti-6Al-4V titanium alloy and human peripheral blood mononuclear cells
✍ Scribed by Maria Martinesi; Sara Bruni; Maria Stio; Cristina Treves; Francesca Borgioli
- Publisher
- John Wiley and Sons
- Year
- 2005
- Tongue
- English
- Weight
- 368 KB
- Volume
- 74A
- Category
- Article
- ISSN
- 1549-3296
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✦ Synopsis
Abstract
The Ti‐6Al‐4V titanium alloy is widely employed as an implant material. The effects of Ti‐6Al‐4V samples, tested in both an untreated state and one in which the samples were subjected to a glow‐discharge treatment performed with the use of air, on human peripheral blood mononuclear cells (PBMC) were studied. Apoptosis, undetectable after 24‐h contact of PBMC with the two sample types, is induced after 48 h by treated samples, and, after 48 h, but in the presence of 1.5 μg/mL PHA, by both sample types. The expression of intercellular adhesion molecule‐1 (ICAM‐1) always increases, in comparison with control, in PBMC put in contact with the two sample types. In the same way, a remarkable increase in tumor necrosis‐alpha (TNF‐α) release in the culture medium is registered, when PBMC are put in contact with the two sample types for 24 and 48 h. Human umbilical‐vein endothelial cells (HUVEC) cocultured for 48 h with PBMC, previously incubated with the two sample types for 24 h, show an increase in ICAM‐1 and vascular cell adhesion molecule‐1 (VCAM‐1) protein expression in comparison with control (HUVEC cocultured with control PBMC), indicating that inflammatory phenomena might occur. Taken together, these results suggest that, although plasma‐treated titanium alloy shows a better biocompatibility in comparison with the untreated one, attention must be paid to the careful control of the first signs of inflammation. © 2005 Wiley Periodicals, Inc. J Biomed Mater Res, 2005