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Cells of the yeast Saccharomyces cerevisiae are transformable by DNA under non-artificial conditions

✍ Scribed by Elke Nevoigt; Anne Fassbender; Ulf Stahl

Publisher: John Wiley and Sons
Year: 2000
Tongue: English
Weight: 87 KB
Volume: 16
Category: Article
ISSN: 0749-503X
DOI: 10.1002/1097-0061(20000915)16:12<1107::aid-yea608>3.0.co;2-3

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✦ Synopsis

Transformants of bakers' yeast (Saccharomyces cerevisiae) can be generated when nongrowing cells metabolize sugars (without additional nutrients) in the presence of plasmid DNA. These results suggest that there is a mechanism by which DNA can naturally be taken up by the yeast cell. Natural transformation does not take place in common complete or minimal yeast culture media such as YPD and YNB. The starvation conditions used in our experiments thus seem to be an important prerequisite for such transformation events.

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