Cell surface glycoproteins I: Accumulation of a glycoprotein on the outer surface of mouse LS cells during mitosis

✍ Scribed by Brown, J. C.

• Book ID: 102438336
• Publisher: Wiley (John Wiley & Sons)
• Year: 1972
• Tongue: English
• Weight: 431 KB
• Volume: 1
• Category: Article
• ISSN: 0091-7419
• DOI: 10.1002/jss.400010102

✦ Synopsis

Surface g l y c o p e p t i d e s d e r i v e d from v e r t e b r a t e c e l l s have been sepa r a t e d i n t o 4 c l a s s e s by chromatography o n DEAE c e l l u l o s e columns.

Among d i f f e r e n t c e l l types t e s t e d , s i g n i f i c a n t d i f f e r e n c e s were observed i n t h e r e l a t i v e amounts o f these 4 g l y c o p e p t i d e c l a s s e s present on t h e c e l l s u r f a c e . T h i s t y p e o f h e t e r o g e n e i t y i s cons i s t e n t w i t h t h e expected b i o l o g i c a l r o l e o f c e l l s u r f a c e g l y c op r o t e i n s . One g l y c o p e p t i d e , as r e v e a l e d by t h e DEAE column a n a ly s i s , was found t o have a c h a r a c t e r i s t i c m e t a b o l i c p a t t e r n i n mouse LS c e l l s .

New accumulation o f t h i s s t r u c t u r e , c a l l e d g l yc o p e p t i d e 4, on t h e c e l l s u r f a c e was d e t e c t e d o n l y around t h e p e r i o d o f c e l l d i v i s i o n (M phase) and n o t a t o t h e r times d u r i n g t h e c e l l c y c l e .