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Cell growth and population activity of Saccharomyces cerevisiae in two-stage continuous cultivation

✍ Scribed by K. Beran; J. Zemanová

Publisher
John Wiley and Sons
Year
1969
Tongue
English
Weight
374 KB
Volume
11
Category
Article
ISSN
0006-3592

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✦ Synopsis

Continuous culture in a cascade of vessels with the addition of supplemental nutrients to any stage permits adjustment of the physiological state of the culture in each stage to best achieve a desired performance goal. The yeast Saccharomyces cerevisiae in two-stage continuous cultivation was selected as a model system. With conditions in the first stage held constant at a selected glucose concentration in the feed stream, dilution rate for the second stage was varied. Cell numbers, dry weight, glucose concentration, respiration coefficient, and titers of several enzymes were determined. The seed rate was defined as the ratio of glucose concentration in the feeds to stage 1 and to stage 2. At low seed rates, the calculated specific growth rate in the second stage was proportional to dilution rate. At higher seed rates, the specific growth rate based on dry weight behaved differently from that based on cell numbers, and the dependence on dilution rate was not linear.

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