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Cell-free synthesis of the Ras-binding domain of c-Raf-1: binding studies to fluorescently labelled H-Ras

โœ Scribed by Jens R Sydor; Ralf P Seidel; Roger S Goody; Martin Engelhard


Book ID
117110933
Publisher
Elsevier Science
Year
1999
Tongue
English
Weight
143 KB
Volume
452
Category
Article
ISSN
0014-5793

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Recognition of Ras by its downstream target Raf is mediated by a Rasrecognition region in the Ras-binding domain (RBD) of Raf. Residues 78-89 in this region occupy two different conformations in the ensemble of NMR solution structures of the RBD: a fully โฃ-helical one, and one where 87-90 form a typ