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Cell-free Protein Synthesis: Methods and Protocols. Edited by Alexander S. Spirin, and James R. Swartz.

✍ Scribed by Takahiro Hohsaka

Publisher
John Wiley and Sons
Year
2008
Tongue
English
Weight
133 KB
Volume
9
Category
Article
ISSN
1439-4227

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## Abstract The use of cell‐free protein synthesis (CFPS) for recombinant protein production is emerging as an important technology. For example, the openness of the cell‐free system allows control of the reaction environment to promote folding of disulfide bonded proteins in a rapid and economical

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A new approach for the regeneration of adenosine triphosphate (ATP) during cell-free protein synthesis was developed to prolong the synthesis and also to avoid the accumulation of inorganic phosphate. This approach was demonstrated in a batch system derived from Escherichia coli. Contrary to the con

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## Abstract Cell‐free translation systems generally utilize high‐energy phosphate compounds to regenerate the adenosine triphosphate (ATP) necessary to drive protein synthesis. This hampers the widespread use and practical implementation of this technology in a batch format due to expensive reagent