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Cell-free protein production and labeling protocol for NMR-based structural proteomics

โœ Scribed by Vinarov, Dmitriy A; Lytle, Betsy L; Peterson, Francis C; Tyler, Ejan M; Volkman, Brian F; Markley, John L


Book ID
109937845
Publisher
Nature Publishing Group
Year
2004
Tongue
English
Weight
380 KB
Volume
1
Category
Article
ISSN
1552-4450

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Cell-free synthesis and amino acid-selec
โœ Takanori Kigawa; Yutaka Muto; Shigeyuki Yokoyama ๐Ÿ“‚ Article ๐Ÿ“… 1995 ๐Ÿ› Springer Netherlands ๐ŸŒ English โš– 536 KB

For the application of multidimensional NMR spectroscopy to larger proteins, it would be useful to perform selective labeling of one of the 20 amino acids. For some amino acids, however, amino acid metabolism drastically reduces the efficiency and selectivity of labeling in in vivo expression system