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Auto-induction medium for the production of [U-15N]- and [U-13C, U-15N]-labeled proteins for NMR screening and structure determination

✍ Scribed by Robert C. Tyler; Hassan K. Sreenath; Shanteri Singh; David J. Aceti; Craig A. Bingman; John L. Markley; Brian G. Fox

Book ID
116828044
Publisher
Elsevier Science
Year
2005
Tongue
English
Weight
688 KB
Volume
40
Category
Article
ISSN
1046-5928

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Protein structure determination by modern NMR techniques is greatly facilitated using 15N-and "C-labeled proteins. Labeling of proteins that are overexpressed in mammalian cells is a difficult task that requires a growth medium consisting of algal hydrolysates supplemented with labeled amino acids.