Counterflow centrifugal elutriation (CCE) has been used to fractionate cell populations on the basis of sedimentation properties, with minimal perturbation of metabolic function. Therefore, it is an ideal method for the isolation of cell cycle phase specific populations. We present modifications of
โฆ LIBER โฆ
Cell-cycle differences of HL-60 leukemia cells fractionated by centrifugal elutriation
โ Scribed by Heil, Matthew F.; Wu, Joseph M.; Chiao, Jen Wei
- Book ID
- 122014977
- Publisher
- Elsevier Science
- Year
- 1985
- Tongue
- English
- Weight
- 272 KB
- Volume
- 845
- Category
- Article
- ISSN
- 0167-4889
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Minor alterations of the electrical circuitry of the centrifuge required for centrifugal elutriation are described. Furthermore it is shown that elutriation can be performed by hydrostatic pressure without the utilization of a pump. This approach enables us to fractionate human blood cells at a cons