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cDNA cloning of the mammalian sterol C5-desaturase and the expression in yeast mutant

✍ Scribed by Shinya Nishi; Hideaki Nishino; Teruo Ishibashi

Book ID
114148684
Publisher
Elsevier Science
Year
2000
Tongue
English
Weight
326 KB
Volume
1490
Category
Article
ISSN
0167-4781

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A yeast null mutant (__erg 3__) defective in __ERG 3__, the gene encoding the C‐5 sterol desaturase required for ergosterol synthesis was transformed with an __Arabidopsis thaliana__ cDNA library inserted in a yeast vector. Transformants (4Γ—10^5^) were screened for cycloheximide (CH) resistance and