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cDNA cloning and gene expression of three small GTP-binding proteins in defense response of chickpea

โœ Scribed by Yuki Ichinose; Kazuhiro Toyoda; Wolfgang Barz


Book ID
114148644
Publisher
Elsevier Science
Year
1999
Tongue
English
Weight
439 KB
Volume
1489
Category
Article
ISSN
0167-4781

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The cloning of small GTP-binding proteins from Petunia hybrida was performed using a PCR-based strategy. Degenerate primers were designed from the DTAGQE and FMETSA consensus sequences. Three different cDNAs were amplified. The deduced polypeptide sequences PhPCRGP1 and PhPCRGP2 were homologous to R