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cDNA cloning and gene expression of ascorbate oxidase in tobacco

✍ Scribed by Naohiro Kato; Muneharu Esaka

Publisher
Springer
Year
1996
Tongue
English
Weight
683 KB
Volume
30
Category
Article
ISSN
0167-4412

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✦ Synopsis

A cDNA clone for ascorbate oxidase (AAO) has been isolated from a cDNA library of tobacco (Nicotiana tabacum) cells. The identity of the amino acid sequence deduced from tobacco AAO cDNA to that from pumpkin AAO cDNA was 68 ~o, which was much lower than the identity (80~o) between pumpkin and cucumber AAO. AAO activity in tobacco cells was much lower than that in pumpkin cells, whereas the immunoreactive protein in tobacco cells was more abundant than that in pumpkin cells. We suppose that AAO protein in tobacco cells may be less active than that in pumpkin cells. Genomic Southern blotting suggested that AAO in tobacco was encoded by a single-copy gene. Northern blotting revealed that mRNA of AAO was highly expressed in young and growing tissues of tobacco plant.

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