cDNA cloning of a tetraubiquitin gene, and expression of ubiquitin-containing transcripts, in aleurone layers ofAvena fatua
✍ Scribed by Gary J. Reynolds; Richard Hooley
- Publisher
- Springer
- Year
- 1992
- Tongue
- English
- Weight
- 561 KB
- Volume
- 20
- Category
- Article
- ISSN
- 0167-4412
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✦ Synopsis
A 2gtll cDNA library, constructed from poly(A) + mRNA isolated from Arena fatua aleurone layers incubated with 1 #M gibberellin A1 (GA1) for 4 days, was screened with an anti-idiotypic antiserum raised against the GA-specific monoclonal antibody MAC 182. One positive clone was isolated, sequenced and shown to encode a tetraubiquitin based on the deduced amino acid sequence. This polyubiquitin cDNA exhibited a high degree of homology to a cloned wheat hexaubiquitin in its 3'-non-coding region. Analysis of total RNA isolated from A. fatua aleurone layers, treated without or with a range of concentrations of GA 1 from 10-11 to 10-6 M, by northern blotting using the cDNA probe revealed 8 different ubiquitin-containing transcript classes all of which are constitutively expressed in aleurone and are regulated by GAl.