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cDNA cloning and characterization of mouse nifS-like protein, m-Nfs1: mitochondrial localization of eukaryotic NifS-like proteins

โœ Scribed by Yumi Nakai; Yoshihiro Yoshihara; Hideyuki Hayashi; Hiroyuki Kagamiyama


Book ID
117109545
Publisher
Elsevier Science
Year
1998
Tongue
English
Weight
376 KB
Volume
433
Category
Article
ISSN
0014-5793

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The cloning of small GTP-binding proteins from Petunia hybrida was performed using a PCR-based strategy. Degenerate primers were designed from the DTAGQE and FMETSA consensus sequences. Three different cDNAs were amplified. The deduced polypeptide sequences PhPCRGP1 and PhPCRGP2 were homologous to R