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cDNA cloning and characterization of a novel gene encoding the MLF1-interacting protein MLF1IP

โœ Scribed by Hanissian, Silva H; Akbar, Umar; Teng, Bin; Janjetovic, Zorica; Hoffmann, Anne; Hitzler, Johann K; Iscove, Norman; Hamre, Kristin; Du, Xiaoping; Tong, Yiai

Book ID
110068525
Publisher
Nature Publishing Group
Year
2004
Tongue
English
Weight
465 KB
Volume
23
Category
Article
ISSN
0950-9232

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The cloning of small GTP-binding proteins from Petunia hybrida was performed using a PCR-based strategy. Degenerate primers were designed from the DTAGQE and FMETSA consensus sequences. Three different cDNAs were amplified. The deduced polypeptide sequences PhPCRGP1 and PhPCRGP2 were homologous to R