CD2: a functional adhesion molecule on murine B cells, involved in interleukin-4-induced aggregation

✍ Scribed by Carina Elenström-Magnusson; Peter Altevogt; Eva Severinson

• Publisher: John Wiley and Sons
• Year: 1993
• Tongue: English
• Weight: 862 KB
• Volume: 23
• Category: Article
• ISSN: 0014-2980
• DOI: 10.1002/eji.1830230418

✦ Synopsis

Abstract

The murine equivalent to CD2, previously known as a T cell marker, is expressed on mouse B cells. The monoclonal anti‐CD2 antibody 12‐15 was found to induce B cell homotypic adhesion. When treated with F(ab')2 fragments of 12‐15, purified, resting B cells aggregate within 2 h of incubation and the response is optimal after 20 h. Anti‐CD2‐induced aggregation is a dose‐related active process, dependent on temperature, metabolic energy and divalent cations. Aggregation is inhibited by two different Fab monomers of anti‐CD2, implying that it is the CD2 molecule itself that functions as an adhesion molecule. We also report that interleukin 4‐induced B cell homotypic adhesion involves CD2‐mediated cell binding, since the antibodies specific for mouse CD2, inhibited interleukin‐4‐induced cell aggregation.