False positiveness in a choline acetyltr
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Y. Kobayashi; Dr. J. de Vellis
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Article
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1985
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John Wiley and Sons
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English
β 275 KB
Dithiothreitol and 2-mercaptoethanol have been frequently used as protective agents of choline acetyltransferase (EC 2.3.1.6) during its purification. We found that those reagents reacted with acetyl-CoA to form products that were coextracted with acetylcholine into organic scintillation liquid to b