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Catalysis of enzyme aggregates in organic solvents. An attempt at evaluation of intrinsic activity of proteases in ethanol

✍ Scribed by Yasuhito Yamamoto; Hideo Kise

Publisher: Springer Netherlands
Year: 1993
Tongue: English
Weight: 396 KB
Volume: 15
Category: Article
ISSN: 0141-5492
DOI: 10.1007/bf00138557

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✦ Synopsis

Catalytic activities of o-chymotrypsin and subtilisin

Car&berg for transesterification of N-acetyl-L-tyrosine methyl ester in ethanol markedly increased by decreasing the amount of the enzymes in the reaction mixtures. The results were kinetically attributed to changes in KM and k,,, due to formation of smaller enzyme aggregates.

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