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Caspase activation in a cardiac cell-free model of apoptosis

✍ Scribed by Claudio Stefaneili; Carla Pignatti; Benedetfa Tantini; Emanuele Giordano; Flavio Flamigni; Claudio Muscari; Carlo Clo; Carlo Guarnieri; Claudio M. Caldarera


Book ID
117141398
Publisher
Elsevier Science
Year
2001
Tongue
English
Weight
177 KB
Volume
33
Category
Article
ISSN
0022-2828

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Caspase-3 activity increased dramatically in cytosolic extracts of rat cerebellar granule cells exposed to apoptotic conditions (basal medium Eagle (BME) containing 5 mM K Ψ‰ without serum) when assayed with Ac-DEVD-amc, but not with Ac-YVAD-afc, a preferred substrate for caspase-1. This provided a b