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Cartilaginous tissue formation from bone marrow cells using rotating wall vessel (RWV) bioreactor

✍ Scribed by Yoshimi Ohyabu; Naoko Kida; Hiroko Kojima; Tetsushi Taguchi; Junzo Tanaka; Toshimasa Uemura

Book ID
101720919
Publisher
John Wiley and Sons
Year
2006
Tongue
English
Weight
316 KB
Volume
95
Category
Article
ISSN
0006-3592

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✦ Synopsis

Abstract

This is the first successful report of the rapid regeneration of three‐dimensional large and homogeneous cartilaginous tissue from rabbit bone marrow cells without a scaffold using a rotating wall vessel (RWV) bioreactor, which simulates a microgravity environment for cells. Bone marrow cells cultured for 3 weeks in DMEM were resuspended and cultured for 4 weeks in the chondrogenic medium within the vessel. Large cylindrical cartilaginous tissue with dimensions of (1.25 ± 0.06) × (0.60 ± 0.08) cm (height × diameter) formed. Their cartilage marker expression was confirmed by mRNA expressions of aggrecan, collagen type I and II, and glycosaminoglycan (GAG)/DNA ratio. Their cartilaginous properties were demonstrated by toluidine blue, safranin‐O staining, and polarization. © 2006 Wiley Periodicals, Inc.

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