The chloride (Cl-) distribution in human red blood cell (RBC) suspensions was studied using cobalt(I1) glycine, [ Co(Gly), 1 -, as a shift reagent (SR). When [ Co(Gly), 1 -was added to suspensions of human RBCs, it shifted the ,'C1 NMR signal of the extracellular C1-. Intracellular and extracellular
β¦ LIBER β¦
Carrier-mediated residual K++and Na++transport of human red blood cells
β Scribed by Karsten Denner; Reinhart Heinrich; Ingolf Bernhardt
- Publisher
- Springer
- Year
- 1993
- Tongue
- English
- Weight
- 869 KB
- Volume
- 132
- Category
- Article
- ISSN
- 0022-2631
No coin nor oath required. For personal study only.
β¦ Synopsis
Residual, i.e., (ouabain, bumetanide, and EGTA)-insensitive K+ and Na+ influxes as well as effluxes of human red blood cells are enhanced in isotonic solutions of low (NaCl + KCl) concentration using sucrose to maintain constant osmolarity. Various carrier models were tested to fit the experimental data of these fluxes simultaneously. The residual K+ and Na+ fluxes can be described on the basis of a carrier mechanism of competing substrates with modifier sites.
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