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Carrier-mediated residual K++and Na++transport of human red blood cells

✍ Scribed by Karsten Denner; Reinhart Heinrich; Ingolf Bernhardt


Publisher
Springer
Year
1993
Tongue
English
Weight
869 KB
Volume
132
Category
Article
ISSN
0022-2631

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✦ Synopsis


Residual, i.e., (ouabain, bumetanide, and EGTA)-insensitive K+ and Na+ influxes as well as effluxes of human red blood cells are enhanced in isotonic solutions of low (NaCl + KCl) concentration using sucrose to maintain constant osmolarity. Various carrier models were tested to fit the experimental data of these fluxes simultaneously. The residual K+ and Na+ fluxes can be described on the basis of a carrier mechanism of competing substrates with modifier sites.


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The chloride (Cl-) distribution in human red blood cell (RBC) suspensions was studied using cobalt(I1) glycine, [ Co(Gly), 1 -, as a shift reagent (SR). When [ Co(Gly), 1 -was added to suspensions of human RBCs, it shifted the ,'C1 NMR signal of the extracellular C1-. Intracellular and extracellular