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Carboxypeptidase Y catalyzed transpeptidations and enzymatic peptide synthesis

✍ Scribed by Klaus Breddam; Fred Widmer; Jack T. Johansen

Publisher
Springer-Verlag
Year
1980
Weight
761 KB
Volume
45
Category
Article
ISSN
0105-1938

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πŸ“œ SIMILAR VOLUMES

Peptide synthesis with immobilized carbo
Peptide synthesis with immobilized carboxypeptidase Y
✍ Steven M. Cramer; Csaba HorvΓ‘th πŸ“‚ Article πŸ“… 1989 πŸ› John Wiley and Sons 🌐 English βš– 886 KB

Enzymatic peptide synthesis was investigated using carboxypeptidase Y immobilized with glutaraldehyde on 10 pm microparticulate amino-silica. Carboxypeptidase Y was immobilized with 98.5% recovery of active enzyme to yield the immobilized enzyme having 0.55 units esterase activity/mg amino-silica su

C-Terminal Labeling of Immunoglobulin G
C-Terminal Labeling of Immunoglobulin G with a Cysteine Derivative by Carboxypeptidase Y Catalyzed Transpeptidation
✍ Shiming Lin; Christopher R. Lowe πŸ“‚ Article πŸ“… 2000 πŸ› Elsevier Science 🌐 English βš– 200 KB

We describe a new method for the site-specific incorporation of an extrinsic cysteine to the C-termini of immunoglobulin G (IgG) using carboxypeptidase Y (CPase Y) catalyzed transpeptidation. The transpeptidase activity of CPase Y was employed to attach cysteine esters to the C-termini of the IgG mo