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Carbohydrate composition of purified human liver α-L-Fucosidase

✍ Scribed by Jack A. Alhadeff; Hud Freeze

Publisher
Springer
Year
1977
Tongue
English
Weight
329 KB
Volume
18
Category
Article
ISSN
0300-8177

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✦ Synopsis

Human liver alpha-L-fucosidase was purified to apparent homogeneity and analyzed for carbohydrate content primarily by gas-liquid chromatography (glc). The enzyme is about 7% carbohydrate by weight and contains the following sugars (residues per 50, 000 molecular weight subunit): mannose (8.3), glucosamine (4.3) (presumably N-acetylated), sialic acid (1.6) and glucose (1.6). Galactose (0.8) and L-fucose (1.8) were also found but their presence may be due to artifacts of the purification procedure.

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