Capillary zone electrophoresis of DNA fragments in a novel polymer network: Poly(N-acryloylaminoethoxyethanol)
β Scribed by Marcella Chiari; Marina Nesi; Prof. Pier Giorgio Righetti
- Publisher
- John Wiley and Sons
- Year
- 1994
- Tongue
- English
- Weight
- 705 KB
- Volume
- 15
- Category
- Article
- ISSN
- 0173-0835
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A noncross-linked interpenetrating polymer network (IPN), consisting of poly(N,N-dimethylacrylamide) (PDMA) and polyvinylpyrrolidone (PVP, weight-average molecular weight M(w) = 1 x 10(6) g/mol) was synthesized by polymerizing N,N-dimethylacrylamide (DMA) monomers directly in PVP buffer solution and
## Abstract Capillary electrophoresis (CE) in entangled polymer solutions was studied using pBR322 DNA Hae III digest as a model sample. The influences of polymer nature, monomer chain length, and polymer concentration were investigated. The use of a novel polymer, galactomannan, as the entangled m
In this article, capillary electrophoresis of DNA fragments and polymerase chain reaction products in sieving liquid polymers is reviewed. The first part of the review is dedicated to the development of N-substituted acrylamides, which in general guarantee a greater resistance to hydrolytic attack.
High speed separation of DNA fragments by CE in