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Capillary SFC-FTIR - effects of the flow-cell on chromatographic resolution

✍ Scribed by Jenkins, Timothy J. ;Kaplan, Muammer ;Davidson, George

Publisher
John Wiley and Sons
Year
1994
Tongue
English
Weight
489 KB
Volume
17
Category
Article
ISSN
0935-6304

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✦ Synopsis

Abstract

The quantitative effects on chromatographic resolution of FTIR flow‐cells for detection in capillary SFC have been determined. A suitably designed cell with a volume of 500 nl was shown to cause only modest broadening of chromatographic peaks obtained from the surfactant mixture Triton X‐100, showing that subsequent detection methods can therefore be used in a multi‐hyphenated chromatographic ensemble. A larger‐volume (980 nl) cell was found to give satisfactory results with 100 μ columns, but not with 50 μm columns. The practicability of a multi‐hyphenated system was illustrated by a preliminary capillary SFC‐UV‐FTIR‐FID study on a plant extract. Good infrared spectra were obtained, together with well‐resolved UV and (subsequent) FID chromatograms.

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