Capillary liquid chromatography/electrospray mass spectrometry for the separation and detection of catechins in green tea and human plasma

✍ Scribed by Joseph J. Dalluge; Bryant C. Nelson; Jeanice B. Thomas; Michael J. Welch; Lane C. Sander

• Publisher: John Wiley and Sons
• Year: 1997
• Tongue: English
• Weight: 102 KB
• Volume: 11
• Category: Article
• ISSN: 0951-4198
• DOI: 10.1002/(sici)1097-0231(19971030)11:16<1753::aid-rcm92>3.0.co;2-g

✦ Synopsis

The separation and detection of biologically active green tea catechins has been accomplished using capillary liquid chromatography/electrospray mass spectrometry (cLC/ESI-MS). Microscale determination ( ~20 ng) of all six catechins in a green tea infusion, and the most extensively studied catechin, (-)epigallocatechin gallate (EGCG), in human plasma is demonstrated by cLC/ESI-MS with selected ion monitoring of protonated molecular ions. The overall quality of the analysis is shown to be dependent on the use of a capillary column with a deactivated, monomeric C 18 stationary phase. The high chromatographic separation efficiency of this packed-capillary column, combined with the high sensitivity and selectivity afforded by the mass spectrometer as detector, provide a reliable approach to the analysis of picomolar quantities of these interesting compounds in complex matrices.