## Abstract Fusedβsilica capillaries chemically derivatized with silane/poly(vinylpyrrolidone) (PVP) or dynamically modified with plexiglas [poly(methyl methacrylate)] were prepared and evaluated with regard to column stability and separation performance for capillary isoelectric focusing of standa
Capillary isoelectric focusing of proteins
β Scribed by Dr. Thomas J. Pritchett
- Publisher
- John Wiley and Sons
- Year
- 1996
- Tongue
- English
- Weight
- 606 KB
- Volume
- 17
- Category
- Article
- ISSN
- 0173-0835
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π SIMILAR VOLUMES
Highly hydrophilic group-substituted celluloses were immobilized on Ε½ . the fused silica capillary inner wall for capillary isoelectric focusing CIEF of proteins. The resultant capillaries were evaluated under typical CIEF conditions, and the results were compared with those obtained by using polyvi
## Abstract A simple method of generating pH gradients for use in capillary isoelectric focusing was described and evaluated experimentally. In the proposed scheme, the cone shaped capillary filled with a conventional buffer is used to produce an effect of nonuniform heating which then generate tem