## Abstract In order to improve precision in protein analysis, a new rinsing procedure with 3M hydrochloric acid was investigated for linear polyacrylamide‐coated capillaries used in isoelectric focusing. After each run the capillaries were rinsed with hydrochloric acid for 5 min, followed by water
Capillary isoelectric focusing: A routine method for protein analysis?
✍ Scribed by Dr. Christine Schwer
- Publisher
- John Wiley and Sons
- Year
- 1995
- Tongue
- English
- Weight
- 505 KB
- Volume
- 16
- Category
- Article
- ISSN
- 0173-0835
No coin nor oath required. For personal study only.
📜 SIMILAR VOLUMES
On-line capillary isoelectric focusing CIEF ᎐electrospray ionization Ž . time-of-flight mass spectrometry ESI᎐TOFMS as a two-dimensional separation Ž system is employed for high-resolution analysis of model proteins myoglobin and . -lactoglobulin and human hemoglobin variants C, S, F, and A. The fo
Capillary isoelectric focusing (CIEF) can provide high-resolution separations of complex protein mixtures, but until recently it has primarily been used with conventional UV detection. This technique would be greatly enhanced by much more information-rich detection methods that can aid in protein ch