Plasma membrane proteins from liver were analysed by concanavalin A affinity and immunoaffinity high-performance liquid chromatography. In the method, four peptide bands with apparent molecular weights of 140,000, 120,000, 80,000 and 60,000 could be isolated. In the second method, with two immobiliz
β¦ LIBER β¦
Capillary High-Performance Liquid Chromatography/Mass Spectrometric Analysis of Proteins from Affinity-Purified Plasma Membrane
β Scribed by Zhao, Yingxin; Zhang, Wei; White, Michael A.; Zhao, Yingming
- Book ID
- 126258105
- Publisher
- American Chemical Society
- Year
- 2003
- Tongue
- English
- Weight
- 132 KB
- Volume
- 75
- Category
- Article
- ISSN
- 0003-2700
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Ultra-high flow rate liquid chromatography on large particle size stationary phases coupled with mass spectrometric detection (particularly tandem mass spectrometry, MS/MS) is gaining increasing usage for the direct determination of pharmaceuticals in biological fluids. The lack of sample preparatio