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Capillary electrophoresis of nucleic acids with a fully automated apparatus

✍ Scribed by Hideko Yamamoto; Takashi Manabe; Tsuneo Okuyama

Publisher
Elsevier Science
Year
1989
Tongue
English
Weight
477 KB
Volume
480
Category
Article
ISSN
1873-3778

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✦ Synopsis

Nucleic acids with a wide range of molecular size, from mononucleotides to calf thymus DNAs, were subjected to capillary electrophoresis employing the conditions for isotachophoretic analysis of proteins; Ampholine was mixed with the sample solution and the electrolyte solutions for isotachophoresis were used. For reproducible analyis, electrophoresis was performed with a fully automated apparatus. Nucleotides were separated from bases owing to their high electrophoretic mobility. The chain length of oligonucleotides was not the major factor with regard to their electrophoretic mobility. Polynucleotides (above lo3 bases) appeared as sharp UV peaks in the zone of the hydrogencarbonate ion and their UV peak width was increased by DNase digestion, suggesting heterogeneity of the product. The applicability of capillary electrophoresis to the separation of large-molecular-sized polynucleotides has been demonstrated.

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