Capillary and slab gel electrophoresis profiling of oligosaccharides

✍ Scribed by Dr. András Guttman; Christopher Starr

• Publisher: John Wiley and Sons
• Year: 1995
• Tongue: English
• Weight: 552 KB
• Volume: 16
• Category: Article
• ISSN: 0173-0835
• DOI: 10.1002/elps.11501601165

✦ Synopsis

Capillary electrophoresis with laser-induced fluorescent detection, a onedimensional version of the well-established planar analytical method of polyacrylamide gel electrophoresis, has been proven to be a powerful new microanalytical method for profiling complex carbohydrates. In this paper a comparison is presented between the planar high concentration polyacrylamide gel electrophoresis method and capillary electrophoresis of different carbohydrates with respect to performance and efficiency. N-Linked oligosaccharides were released from several glycoproteins, including fetuin, human immunodeficiency virus (HIV) envelope recombinant glycoprotein (GP-120), a,-acid glycoprotein and ribonuclease B, using recombinant peptide-N-glycosidase F (PNGase F). Both separation methods involve labeling of the released carbohydrates at the reducing end with the fluorescent dye, disodium 8-amino-1,3,6naphthalene trisulfonate (ANTS). Fluorophore labeling was followed by separation of the labeled oligosaccharides either by high concentration polyacrylamide gel electrophoresis or capillary electrophoresis.