Candidate chromosomal regions for genes involved in activation of alternative lengthening of telomeres in human immortal cell lines
β Scribed by Nobumasa Shigeeda; Minoru Uchida; J.Carl Barrett; Takeki Tsutsui
- Book ID
- 114253904
- Publisher
- Elsevier Science
- Year
- 2003
- Tongue
- English
- Weight
- 369 KB
- Volume
- 38
- Category
- Article
- ISSN
- 0531-5565
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We have previously reported that continuous in vitro passage in the presence of 3T3 feeders of a non-tumorigenic adenoma-derived epithelial cell line, designated PCIAA, resulted in its becoming immortal. At early passage PCIAA was normal diploid, whereas every cell of PCIAA late passage had an isoch
A non-tumorigenic epithelial cell line designated PCIAA, derived from a large pre-malignant colorectal adenoma from a patient with familial polyposis coli (also referred to as hereditary adenomatosis of the colon and rectum) has become immortal in vitro. PCIAA has been passaged in vitro continuously
## Abstract ## Background We modified a tetracyclineβregulated system that can control the activity of individual genes quantitatively and reversibly in transgenic mammals. Despite these advances, there remained one problem in the intensive use of the tetβsystem: the limited range of acceptor cell