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Cancer procoagulant in serum of rats during development of experimental epithelioma

✍ Scribed by W. Mielicki; R. Wierzbicki


Publisher
John Wiley and Sons
Year
1990
Tongue
French
Weight
244 KB
Volume
45
Category
Article
ISSN
0020-7136

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✦ Synopsis


Abstract

The activity of cancer procoagulant (CP) during the development of GuΓ©rin epithelioma was studied in the blood of Wistar rats. Blood was collected from the carotid artery and, after clotting, proteins adsorbing on aluminum hydroxide were removed from the serum. Then procoagulant activity was determined in the test system without factor VII by means of substrate S‐2222 specific for factor Xa. A statistically significant increase in the activity of CP in serum was detected, coinciding with the period of intensive tumor growth (15th–25th day of disease).


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## Abstract Mouse blastocysts cultured in vitro in rat serum develop through the early somitic stages of embryogenesis at the same rate as embryos cultured in human cord serum. Rat serum can thus be used instead of human cord serum for in vitro culturing of mouse embryos.