## Abstract Poly(Lys^48^, His^52^), a random copolypeptide of Lβlysine (48%) and Lβhistidine (52%), was used as a model protein for investigating the effects of protonation on the imidazole group of histidines on protein binding to DNA. The complexes formed between poly(Lys^48^, His^52^) and DNA we
Calorimetric studies of the interaction between dna and poly-l-lysine
β Scribed by H. Klump
- Publisher
- Elsevier Science
- Year
- 1976
- Tongue
- English
- Weight
- 417 KB
- Volume
- 5
- Category
- Article
- ISSN
- 0301-4622
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Interaction between polylysine and DNA's of varied G + C contents was studied using thermal denaturation and circular dichroism (CD). For each complex there is one melting band at a lower temperature t,,, corresponding to the helix-coil transition of free base pairs, and another band a t a higher te
## Abstract Interaction between poly(Lys^50^, Tyr^50^) and DNA has been studied by absorption, circular dichroism (CD), and fluorescence spectroscopy and thermal denaturation in 0.001__M__ Tris, pH 6.8. The binding of this copolypeptide to DNA results in an absorbance enhancement and fluorescence q
The surface force apparatus has been used to study the force law between mica surfaces modified with the biopolymers heparin and poly-L-lysine by direct adsorption from solution. Between identical surfaces the interactions were well described by the DLVO theory for repulsive double-layer overlap. Be
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