Calmodulin as a modulator of NaCl transport in the posterior salt-transporting gills of the Chinese crabEriocheir sinensis

✍ Scribed by A. Péqueux; R. Gilles

• Publisher: Springer-Verlag
• Year: 1992
• Tongue: English
• Weight: 551 KB
• Volume: 113
• Category: Article
• ISSN: 0025-3162
• DOI: 10.1007/bf00367639

✦ Synopsis

The possible involvement of calmodulin-dependent processes in the control of Na + and C1-transport pathways has been investigated on isolated, perfused preparations of salt-transporting posterior gills of the euryhaline Chinese crab Eriocheir sinensis (collected near Emden, Germany in autumn 1990). The anti-calmodulin phenothiazine drugs Chlorpromazine and Trifluoperazine induced depolarization of the transepithelial potential only when added to the serosal bathing saline (socalled "in"). This effect is best interpreted by assuming a disturbance of the conductive C1-pathways located at the baso-lateral side of the epithelium. In agreement with that conclusion is the fact that Trifluoperazine inhibits the C1-transepithelial influx. Trifluoperazine also induces inhibition of the Na + influx when added either to the incubation (out) or to the perfusion (in) medium. These results indicate inhibitory effects of the anticalmodulin drug on both the Na+/K + pump and leak system located at the serosal side and on the Na+/H + exchange located at the apical side of the epithelium.