Immunological identification and biochemical characterization of a glucose transporter in the posterior gills of the crabCarcinus maenas (L.)

✍ Scribed by Welcomme, L. ;Devos, P.

• Publisher: John Wiley and Sons
• Year: 1994
• Tongue: English
• Weight: 637 KB
• Volume: 268
• Category: Article
• ISSN: 0022-104X
• DOI: 10.1002/jez.1402680306

✦ Synopsis

Abstract

The euryhaline crab Carcinus maenas when adapted to hyposmotic medium shows an ion transporting‐type epithelium in the three posterior gills. When they are perfused in hyposmotic conditions, there is highly specific sodium‐independent glucose transport activity. This saturable transport activity is inhibited by phloretin and diethylstilbestrol.

Immunological data obtained with the utilization of the antibody RaIRGT (antibody anti‐glucose transporter found in rat adipocytes) reveals the existence of a single bound protein of 38 Kd. This protein can specifically bind to cytochalasin B which has a high affinity for the glucose transporter found in numerous tissues of vertebrates.

These biochemical and immunological data point to the existence of a glucose transporter of 38 Kd in the branchial tissue of this species of crab. © 1994 Wiley‐Liss, Inc.