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Callus production from willow (Salix viminalisL.) protoplasts

โœ Scribed by Tiina Vahala; Tage Eriksson

Book ID
104647801
Publisher
Springer
Year
1991
Tongue
English
Weight
662 KB
Volume
27
Category
Article
ISSN
0167-6857

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โœฆ Synopsis

Protoplasts were isolated from cell suspensions of Sal& viminal& basket willow) clone 78-0-90 and S. schwerinii clone 77-0-77, using cellulysin and macerase in modified Woody Plant medium. For clone 78-0-90, 6.3.106 -+ 1.9.106 protoplasts were obtained per gram flesh weight. Cell divisions started two days after protoplast isolation and gave rise to callus which has been maintained in culture for up to four years. Protoplast yield from the clone 77-0-77 was lower (less than 106 protoplasts per gram cells), cell division was infrequent and no callus was obtained. Protoplasts were also isolated from the leaves of willow shoot cultures using cellulysin and pectolyase, but these did not show cell divisions.

๐Ÿ“œ SIMILAR VOLUMES

Inhibitor content of phloem and xylem sa
Inhibitor content of phloem and xylem sap obtained from willow (Salix viminalisL.) entering dormancy
โœ M. R. Bowen; G. V. Hoad ๐Ÿ“‚ Article ๐Ÿ“… 1968 ๐Ÿ› Springer-Verlag ๐ŸŒ English โš– 301 KB

It is shown that there is one growth inhibitor in the phloem sap and two in the xylem sap of willow (S. viminalis L.). The concentration of the inhibitor in the phloem sap, (+)-abscisie acid, increases as the plants enter dormancy. This is Mso shown for (+)-abscisic acid in the xylem sap, hut the co