𝔖 Bobbio Scriptorium
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Callus formation from protoplasts of culturedSpinacia oleraceacells

✍ Scribed by H. Nakagawa; H. Tanaka; T. Oba; N. Ogura; M. Iizuka


Publisher
Springer
Year
1985
Tongue
English
Weight
639 KB
Volume
4
Category
Article
ISSN
0721-7714

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Callus formation from root protoplasts o
✍ M. Brison; A. Lamant πŸ“‚ Article πŸ“… 1990 πŸ› Springer 🌐 English βš– 512 KB

Root protoplasts of Quercus rubra L. were isolated from 12 day old seedlings with an enzyme mixture containing Cellulase R1O + Rhozyme HP150 + Macerozyme R1O, supplemented with cysteine and bovine serum albumin.Protoplasts were purified by a Ficoll density gradient centrifugation and cultured at low