## An ultrasensitive protein assay, linear between 0.01 and 0.2 pg protein, is described. In this assay, copper is complexed to protein, excess copper is removed by adsorption to a small Sephadex column, and the copper-protein complex is destroyed by digestion with hydroperoxide. Phenol and chlor
Calibration of Colorimetric Protein Assays for Quantitation of Plant AB Toxins
✍ Scribed by Marta Jiménez; Sabine André; Hans-Joachim Gabius; Dolores Solı́s
- Publisher
- Elsevier Science
- Year
- 2000
- Tongue
- English
- Weight
- 66 KB
- Volume
- 284
- Category
- Article
- ISSN
- 0003-2697
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✦ Synopsis
Analysis of patterns and quantitation of genes are some the most important methods in cell biology, biochemistry, and molecular biology as well as in clinical science. The expression of individual genes can be detected conventionally by Northern blot analysis and other related methods (1). These procedures require relatively large amounts of RNA. More recently, the
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A modified bromosulfalein assay for the quantitative estimation of protein is described that is simple, rapid, and sensitive. The assay can be used in the range between 0.5 and 100 ~g of protein with samples containing from about 0.01 to 5 mg of protein/ml. Estimates of protein levels in,homogenates