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Calibration of a luminescence photometer for the purpose of obtaining corrected fluorescence spectra

โœ Scribed by I. I. Solov'eva; V. V. Firyulina; L. A. Shiffers; N. N. Grigor'ev; Ya. S. Polyakov; L. A. Solov'eva; D. B. Gladilovich; K. P. Stolyarov

Book ID
104870933
Publisher
Springer US
Year
1985
Tongue
English
Weight
435 KB
Volume
42
Category
Article
ISSN
0021-9037

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Background: Numerous applications of fluorescence microscopy require quantitation of signal intensity in reproducible units. Two problems must be overcome to achieve this goal. First, due to various instrumental factors, the same sample imaged on two microscopes or even on the same microscope at dif