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Calibration mixture for estimation of peptide molecular weight by exclusion chromatography

โœ Scribed by Harvey Wilgus; Earle Stellwagen

Publisher
Elsevier Science
Year
1979
Tongue
English
Weight
207 KB
Volume
94
Category
Article
ISSN
0003-2697

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โœฆ Synopsis

A procedure is described for the rapid preparation of a partial cyanogen bromide digest of horse heart cytochrome c. This digest serves as a calibration mixture for estimation of pep tide molecular weight in the range 500 to 20,000 by exclusion chromatography in denaturing solvents. The elution profile of the calibration mixture from Sephadex G-50 equilibrated and developed with 10% formic acid is described by the relationship log M = 4.79 -0.42

Methods

Superfine Sephadex G-50 was purchased from Pharmacia, Inc. A 5 x 150-cm column was equilibrated with 10% (v/v) formic acid

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