Molecular characterization of poly(diisopropyl fumarate) by the absolute calibration method in molecular exclusion chromatography (GPC)
✍ Scribed by Cortizo, M. S. ;Andreetta, H. A. ;Figini, R. V.
- Publisher
- John Wiley and Sons
- Year
- 1989
- Tongue
- English
- Weight
- 287 KB
- Volume
- 12
- Category
- Article
- ISSN
- 0935-6304
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✦ Synopsis
The present work demonstrates that it is possible to obtain the parameters Kand a of the Staudinger-Mark-Houwink relationship between the intrinsicviscosity [q] and the molecularweight Mof a polymer by applying the absolute method of exclusion chromatographyto samples of poly(diisopropy1 fumarate).The procedure is based on deducing the relationship between molecular weight and elution volume V from chromatographic runs of a stoichiometrically labeled polymer sample with a broad molecular weight distribution. By using double detection it is possible to obtain the relationship f(V)/h(V) =M(V)/M, =exp (A-5V)/Mn where M,, is the osmotically determined number average of the molecular weight oftheeluted polymerwhile f(ll)and h(V)arethenormalized elution curves obtained by the use of the polymer mass detector and the label detector respectively. A and 5 are the parameters of the calibration curve, Le., the relationship between M and V which togetherwith the intrinsicviscosity and the elution curves of several samples of the polymer allows us to obtain the relationship between [q] and M.
The results have beenverified with chromatographic data through the use of the universal calibration concept.