Calcium signal induced by mechanical perturbation of osteoclasts

✍ Scribed by Shen-Ling Xia; Jack Ferrier

• Book ID: 102886494
• Publisher: John Wiley and Sons
• Year: 1995
• Tongue: English
• Weight: 983 KB
• Volume: 163
• Category: Article
• ISSN: 0021-9541
• DOI: 10.1002/jcp.1041630309

✦ Synopsis

Abstract

Multinucleated osteoclasts from rabbit long bone, 1–6 days in culture, respond to mechanical perturbation with a transient increase of intracellular calcium concentration ([Ca^2+^]~i~), as measured with the fluorescent indicator fluo‐3 on a confocal laser scanning microscope. In experiments with different extracellular calcium concentrations (from 11.8 mM to calcium‐free), the incidence, the magnitude, and the duration of [Ca^2+^]~i~ responses decreases with decreasing bathing [Ca^2+^]. Following mechanical perturbation, a thapsigargin‐induced [Ca^2+^]~i~ response has a lower magnitude than the thapsigargin‐induced response without mechanical perturbation. In thapsigargin‐pretreated osteoclasts the mechanical perturbation‐induced rise in [Ca^2+^]~i~ is larger and longer than in control cells. Ni^2+^ inhibits the incidence and decreases both the magnitude and the duration of the responses, while nifedipine, verapamil, and Gd^3+^ have no effect. These measurements show that rabbit osteoclasts transduce a mechanical perturbation of the cell membrane into a [Ca^2+^]~i~ signal via both a calcium influx and an internal calcium release. © 1995 Wiley‐Liss, Inc.