Calcium, cells and bone
- Publisher
- Springer
- Year
- 1991
- Tongue
- English
- Weight
- 126 KB
- Volume
- 9
- Category
- Article
- ISSN
- 0914-8779
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## Abstract Bone cells were isolated by collagenaseβhyaluronidase digestion of 19β20 dayβold fetal rat calvaria. It is shown that contamination from calcified matrix can give erroneously high values for total cell calcium and can mask intracellular calcium exchange. Filtration of the cell suspensi
## Abstract Calcium transport was studied in bone cells isolated from fetal rat calvaria. ^45^Ca uptake experiments revealed an active component of calcium exchange. Calcium uptake was inhibited by iodoacetamide, DNP, CCCP and oligomycin and appeared to be dependent on medium phosphate concentratio
## Abstract Bone disease with osteoporosis and osteomalacia are common in sickle cell disease (SCD). Some patients have vitamin D deficiency and low bone mineral density (BMD). The role of vitamin D and calcium supplementation to restore bone health in SCD has not been well studied. In 14 adults wi