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Caffeine enhancement of digestion of DNA by nuclease S1

โœ Scribed by C.J. Chetsanga; K. Rushlow; V. Boyd


Book ID
113191004
Publisher
Elsevier Science
Year
1976
Tongue
English
Weight
585 KB
Volume
34
Category
Article
ISSN
0027-5107

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An improved method for the isolation of the inverted repetitive (foldback) sequences present in mammalian DNAs is described. It makes use of the new observation that nuctease Sl digestion of denatured DNA occurred at a faster rate and was more extensive in medium containing dioxaue. The temperature-